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CXCR4/ SDF-1α对宫颈癌Hela细胞定向迁移及增殖的影响
发表时间：2008-08-16 发表者：张建平
(访问人次：546)
【摘要】 目的 了解CXCR4在Hela细胞的表达情况，并借助细胞培养评价CXCR4/ SDF-1α对Hela细胞定向迁移及增殖的影响。方法 CXCR4 mAb免疫染色Hela细胞。用Transwell侵袭转移模型评价Hela细胞的迁移情况，其中，上室中加入预先用（或不用）CXCR4单抗预孵育的Hela细胞，下室中加入含0～100ng/ml SDF-1α的培养基。为评价CXCR4、SDF-1α对Hela细胞增殖的影响，将Hela细胞接种于有(无)SDF-1α和（或）CXCR4的低血清环境72小时。结果 CXCR4在所有Hela细胞上均有表达。Hela细胞能顺SDF-1α浓度差定向迁移，且这一作用可被CXCR4 mAb拮抗。SDF-1α能促进Hela细胞在低血清环境中增殖。结论 CXCR4/ SDF-1α参与了Hela细胞定向迁移的过程并影响其增殖。
【关键词】 Hela细胞株；定向迁移；增殖；CXCR4；SDF-1α
中图分类号：R737.3
Effect of CXCR4/ SDF-1α in derected migration and proliferation of Hela cells.
ZHANG Jian-ping1, LU Wei-guo2, XIE Xing2.
1.Department of Obstetrics and Gynecology, The First Hospital Affiliated to Suzhou University, Suzhou 215006,China
2.Department of Gynecologic Oncology, Women’s Hospital, School of Medicine, Zhejiang University, Hangzhou 310006, China
Corresponding author: ZHANG Jian-ping, Email: g5615335@126.com
【Abstract】 Objective To investigate the expression of CXCR4 in Hela cells, and evaluate the effect of CXCR4/ SDF-1α in directed migration and proliferation of Hela cells. Methods Hela cells were immunohistochemically stained with mouse anti-CXCR4 monoclonal antibody (mAb). In vitro invasion of Hela cells was evaluated using Transwell Permeable Supports, in which Hela cells with/without CXCR4 mAb pre-incubation were seeded in the upper chambers, medium containing 0-100ng/ml of SDF-1α was added to the lower compartments. For evaluating the effect of CXCR4/ SDF-1α on proliferation of cervical cancer cells, Hela cells were cultured for 72 hours exposed to SDF-1α with and without CXCR4 mAb. Results CXCR4 was expressed on all Hela cells. SDF-1α induced the directed migration of Hela cells with a concentration-depended model, which was inhibited by using CXCR4 mAb. SDF-1α also stimulated the proliferation of Hela cells mediated by CXCR4. Conclusion CXCR4/ SDF-1α participates in the directed migration and proliferation of Hela cells.
【Key words】 Hela cell line; directed migration; proliferation;CXCR4; SDF-1α
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