CXCR4/ SDF-1α implied in many cancer metastatic mechanism. Cervical squamous cell cancer (SCC) tissues (n=35), normal cervical tissues (n=10), metastatic (n=10) and nometastatic lymph nodes (n=50), and Hela cells were stained immunohistochemically with CXCR4 MoAb. Meanwhile, lymph nodes were stained immunohistochemically with rabbit anti-SDF-1α. In vitro invasion of Hela cells was evaluated using Transwell Permeable Supports, in which Hela cells with/without CXCR4 mAb pre-incubation were seeded in the upper chambers, medium containing 0-100ng/ml SDF-1α was added to the lower compartments. For evaluating the effect of CXCR4/ SDF-1α on proliferation of cervical cancer cells, Hela cells were cultured for 72 hours exposed to SDF-1α with and without CXCR4 mAb. We found CXCR4 was expressed on SCC cells in all cervical cancer, metastatic lymph node and Hela cells, but not in normal cervix. SDF-1α was expressed on lymph cells in all lymph nodes. SDF-1α induced the directed migration of Hela cells with a concentration-depended model, which was inhibited by CXCR4 mAb. SDF-1α also stimulated the proliferation of Hela cells mediated by CXCR4. CXCR4/ SDF-1α axis probably participates in the metastasis towards lymph nodes in cervical cancer.